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This sheet was compiled from PubMed (NIH) abstracts using AI assistance. Every factual claim is cited to a real PubMed article (see the source list). It has not yet been human-reviewed — confirm with a healthcare provider before use.
Compiled from 30 PubMed articles · model: gemma4:31b
Summary
Inonotus obliquus, known as Chaga, is a medicinal mushroom used since the sixteenth century for various therapeutic purposes [PMID:38813471]. Modern research indicates it possesses antioxidant, anti-inflammatory, anticancer, and anti-diabetic properties [PMID:38813471, PMID:38116085, PMID:39170453].
Background
Chaga (Inonotus obliquus) is a parasitic fungus that grows on birch trees [PMID:29484963]. It is characterized as a medicinal mushroom and functional food with high nutritional value [PMID:37513265, PMID:19407959].
Traditional uses
Traditional applications include the treatment of gastrointestinal cancer, diabetes, bacterial infections, liver diseases [PMID:38813471], breast, cervix, and skin cancers [PMID:38116085], gastritis, gastric ulcers, and tuberculosis of the bones [PMID:23149251, PMID:32877719]. It has also been used in pipe smoking rituals and mystical future forecasts [PMID:38116085].
Active compounds
Bioactive compounds include polysaccharides (specifically Inonotus obliquus polysaccharide or IOPS) [PMID:33947037], triterpenoids (such as inotodiol, betulin, lanosterol, ergosterol, and $\beta$-sitosterol) [PMID:35335249, PMID:40243601], polyphenols, flavonoids, steroids, and lignin metabolites [PMID:38813471, PMID:38116085, PMID:39202879].
Mechanism of action
Mechanisms include the suppression of iNOS and NO production via lanostanoids [PMID:33434790], regulation of the NOS-cGMP-PDE5 signaling pathway for glucolipid metabolism [PMID:39059831], and induction of autophagy via the AMPK-mTOR pathway in breast cancer cells [PMID:33798660]. It may also act by inhibiting energy metabolism (glycolysis and mitochondrial respiration) and suppressing STAT3 activity in oral cancer cells [PMID:38720012], and regulating Nrf2 signaling to exert antioxidative effects [PMID:30878614].
Clinical evidence
Evidence DBladder Cancer (Dog Organoids)
Chaga extract inhibited cell viability in a concentration-dependent manner, arrested the cell cycle, and induced apoptosis [PMID:37153767].
Evidence DBreast Cancer (Mice/In Vitro)
Oral administration of Chaga mushroom extract (2 g/kg) demonstrated anti-tumor effects in 4T1 tumor-bearing BALB/c mice [PMID:33798660].
Evidence DOral Cancer (In Vitro)
Extract reduced cell viability and proliferation of HSC-4 cells by inhibiting glycolysis and mitochondrial membrane potential [PMID:38720012].
Evidence DRenal Fibrosis (Mice)
Chaga ameliorates folic acid-induced renal fibrosis in mice [PMID:40160460].
Evidence DType 2 Diabetes (Rats)
Chaga extracts (50 and 100 mg/kg) were used to treat glucolipid metabolism disorders and abnormal renal functions in HFD/STZ-induced T2DM rats [PMID:39059831].
Evidence DAtopic Dermatitis (Mice)
Safety & adverse effects
A case report identified oxalate nephropathy (diffuse tubular atrophy and interstitial fibrosis) in a patient ingesting 4-5 teaspoons of Chaga mushroom powder daily, noting that Chaga contains extremely high oxalate concentrations [PMID:23149251]. Additionally, wild chaga conks have been found to contain high activity concentrations of 40K and mild contamination with 137Cs [PMID:37089251].
Evidence summary
The current evidence is primarily based on in vitro studies, animal models (mice/rats), and a single case report. There is a lack of human clinical trials (RCTs) or systematic reviews of clinical data, placing the overall strength of clinical evidence at level D.
PubMed sources
1.PMID: 38813471 (2024) — Therapeutic properties of Inonotus obliquus (Chaga mushroom): A review. · Mycology
2.PMID: 38116085 (2023) — Chaga mushroom: a super-fungus with countless facets and untapped potential. · Frontiers in pharmacology
3.PMID: 37513265 (2023) — Medicinal Mushrooms: Their Bioactive Components, Nutritional Value and Application in Functional Food Production-A Review. · Molecules (Basel, Switzerland)
4.PMID: 35335249 (2022) — Supercritical CO2 Extraction of Triterpenoids from Chaga Sterile Conk of Inonotus obliquus. · Molecules (Basel, Switzerland)
5.PMID: 37153767 (2023) — Anti-cancer activity of Chaga mushroom (Inonotus obliquus) against dog bladder cancer organoids.
Government sources
No direct government monograph is available for this herb. The content below is AI-generated and has not been verified against an authoritative government source. Use the search links to check official sources before relying on this information.
7.PMID: 40160460 (2025) — Inonotus obliquus (chaga) ameliorates folic acid-induced renal fibrosis in mice: the crosstalk analysis among PT cells, macrophages and T cells based on single-cell sequencing. · Frontiers in pharmacology
8.PMID: 39170453 (2024) — A brief overview of the medicinal and nutraceutical importance of Inonotus obliquus (chaga) mushrooms. · Heliyon
12.PMID: 34195023 (2021) — Inonotus obliquus - from folk medicine to clinical use. · Journal of traditional and complementary medicine
13.PMID: 33639077 (2021) — Medicinal Mushrooms against Influenza Viruses. · International journal of medicinal mushrooms
14.PMID: 33800424 (2021) — Comparison of Polysaccharides Extracted from Cultivated Mycelium of Inonotus obliquus with Polysaccharide Fractions Obtained from Sterile Conk (Chaga) and Birch Heart Rot. · Journal of fungi (Basel, Switzerland)
15.PMID: 39059831 (2024) — Inonotus obliquus (Chaga) against HFD/STZ-induced glucolipid metabolism disorders and abnormal renal functions by regulating NOS-cGMP-PDE5 signaling pathway. · Chinese journal of natural medicines
16.PMID: 40868987 (2025) — Molecular Mechanisms of Phytochemicals from Chaga Mushroom (Inonotus obliquus) Against Colorectal Cancer: Insights from Network Pharmacology, Molecular Docking, and Bioinformatics. · International journal of molecular sciences
17.PMID: 19407959 (2009) — Progress of research on Inonotus obliquus. · Chinese journal of integrative medicine
19.PMID: 39202879 (2024) — Impact of Incorporating Dried Chaga Mushroom (Inonotus obliquus) into Gluten-Free Bread on Its Antioxidant and Sensory Characteristics. · Molecules (Basel, Switzerland)
20.PMID: 38720012 (2024) — Chaga mushroom extract suppresses oral cancer cell growth via inhibition of energy metabolism. · Scientific reports
22.PMID: 38046279 (2023) — Chaga mushroom triterpenoids as adjuncts to minimally invasive cancer therapies: A review. · Current research in toxicology
23.PMID: 32877719 (2021) — Deciphering the antitumoral potential of the bioactive metabolites from medicinal mushroom Inonotus obliquus. · Journal of ethnopharmacology
24.PMID: 40508014 (2025) — Elucidating the Anti-Diabetic Mechanisms of Mushroom Chaga (Inonotus obliquus) by Integrating LC-MS, Network Pharmacology, Molecular Docking, and Bioinformatics. · International journal of molecular sciences
25.PMID: 33798660 (2021) — Chaga mushroom extract induces autophagy via the AMPK-mTOR signaling pathway in breast cancer cells. · Journal of ethnopharmacology
26.PMID: 40243601 (2025) — Comparative Study of Chaga (Inonotus obliquus) Dietary Supplements Using Complementary Analytical Techniques. · International journal of molecular sciences
27.PMID: 41581894 (2026) — Chaga Mushroom (Inonotus obliquus) Attenuates DNCB-Induced Atopic Dermatitis by Modulating Oxidative Stress and Cytokine Expression. · Journal of microbiology and biotechnology
28.PMID: 30878614 (2019) — Inonotus obliquus polysaccharides protect against Alzheimer's disease by regulating Nrf2 signaling and exerting antioxidative and antiapoptotic effects. · International journal of biological macromolecules
29.PMID: 27562990 (2016) — [Composition and biological activity of triterpenes and steroids from Inonotus obliquus (chaga)]. · Biomeditsinskaia khimiia
30.PMID: 29484963 (2018) — Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B). · Integrative cancer therapies